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Fluorophores Excitation Emission Spectra. The potential extension of our approach to even more fluorophores may be achieved by further increasing the number of excitation wavelengths or integrating emission dispersion Whereas in this work we focused on a facile system based on a lamp-operated epifluorescence microscope the fast multi-fluorophore and quantitative biosensor imaging. Example of APC properties and filterbandpass used for data acquisition. Fluorophores can absorb and emit light within a range of wavelengths normally referred to as the absorbance excitation and emission spectra. Each fluorophore has its specific spectral characteristics with excitation and emission spectra particular to the molecule.
The emission maximum is chosen and only emission light at that wavelength is allowed to pass to the detector. Fluorophores or fluorochromes are commonly used in conjugation with antibodies as detection reagents in applications such as flow cytometry. The potential extension of our approach to even more fluorophores may be achieved by further increasing the number of excitation wavelengths or integrating emission dispersion Whereas in this work we focused on a facile system based on a lamp-operated epifluorescence microscope the fast multi-fluorophore and quantitative biosensor. Effect of excitation at different wavelengths on the fluorophore emission. Compare the excitation and emission parameters for many fluorophores to find compatible reagents and optimal filters for your experiment. FLUOROPHORE TABLE Dye Absorbance Wavelength Emission Wavelength Visible color Hydroxycoumarin 325 386 blue methoxycoumarin 360 410 blue Alexa fluor 345 442 blue aminocoumarin 350 445 blue Cy2 490 510 green dark FAM 495 516 green dark Alexa fluor 488 494 517 green light Fluorescein FITC 495 518 green light Alexa fluor 430 430 545 green light.
The lambda max for the excitation of this fluorophore is 650 nm while the emission is 660 nm.
Effect of excitation at different wavelengths on the fluorophore emission. This page works best in IE9 or higher Firefox or Chrome. This tandem dye is known by a confusing myriad of. Compare the excitation and emission parameters for many fluorophores to find compatible reagents and optimal filters for your experiment. Fluorescent probes or fluorophores fluorescent dyes or proteins are coupled to a secondary antibody or streptavidin to allow visualization of an analyte. FLUOROPHORE TABLE Dye Absorbance Wavelength Emission Wavelength Visible color Hydroxycoumarin 325 386 blue methoxycoumarin 360 410 blue Alexa fluor 345 442 blue aminocoumarin 350 445 blue Cy2 490 510 green dark FAM 495 516 green dark Alexa fluor 488 494 517 green light Fluorescein FITC 495 518 green light Alexa fluor 430 430 545 green light.