24
Sep
Human Neutrophil Isolation Kit. This cocktail contains antibodies against CD2 CD3 CD9 CD19 CD36 CD56 and glycophorin A. Many methods are available for isolating neutrophils from human peripheral blood including density gradient centrifugation and both positive and negative magnetic selection eg see Chapters 3 and 4 of this volume. EasySep Human Neutrophil Enrichment Kit - Immunomagnetic isolation of untouched human neutrophils. Mix gently by inverting tubes seven to eight times.
22 Alternatively neutrophils were isolated from EDTA-anticoagulated whole blood by using the EasySep. The benefits of this kit include. Many methods are available for isolating neutrophils from human peripheral blood including density gradient centrifugation and both positive and negative magnetic selection eg see Chapters 3 and 4 of this volume. Briefly isolation cocktail 50. Dead cells were excluded by Helix NP Blue. Neutrophils were isolated from consenting healthy adult donors under Brigham and Womens Hospital protocol 2006P001068 by red blood cell sedimentation with dextran 3 followed by Ficoll-Hypaque density gradient centrifugation and hypotonic erythrocyte lysis as has been described.
Final purity and yield may vary between different donors.
This cocktail contains antibodies against CD2 CD3 CD9 CD19 CD36 CD56 and glycophorin A. Mix gently by inverting tubes seven to eight times. Isolation of human neutrophils from whole blood was performed by the gradient density centrifugation method. Start cell samples are prepared by performing a HetaSep. The neutrophils were stored in -80 C before DNA extractions. Briefly isolation cocktail 50.