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Spe1 Restriction Site Sequence. Single-buffer simplicity means more straightforward and streamlined sample processing. Recognition Sites TCTAGA indicates methylation sensitivity. 1086A for complete product documentation and resources. Cleavage followed by fill-in of 5 overhangs to generate blunt ends.
Thermo Scientific SdaI SbfI restriction enzyme recognizes CCTGCAGG sites and cuts best at 37C in its own unique buffer. This plasmid is available through Addgene. Promoter T7 Tag Fusion Protein. The sequence in green is a Pst1 restriction site and that in orange is the Spe1 restriction site. These new restriction sites may be generated by. The region in purple is the sequence of shared overlap with the forward primer.
Click Sequence Details to view all sequence information for this locus including that for other strains.
Welcome to RestrictionMapper - on line restriction mapping the easy way. Plasmid pMJ915v2Nterm Spe1 Cterm Age1 site from Dr. Cleavage with two restriction endonucleases that produce blunt ends. Sequence Help SPE1 YKL184W Sequence Sequences and genome annotation information for Reference Strain S288C and a select set of Alternative References. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction. Sequence of DNA recognized by the enzyme and to which it specifically binds.