20
Jul
Syto 9 Excitation Emission. SYTO 9DNA Emission Spectra. With SYTO dyes generally show cytoplasmic or mi to chon dri al staining as well as nuclear staining. Excitation filter Dichroic mirror Emission filter Remarks. Excitation and Emission Spectra To establish emissionexcitation spectra SYTO9 and PI were added to solutions of DNA.
For SYTO9 emission spectra were collected with excitation at 420 nm and the absorption spectrum was measured by emissions at 580 nm. The excitationemission maxima for these dyes are about 480500nm for SYTO 9 stain and 490635nm for propidium iodide. Propidium iodide or PI is a fluorescent intercalating agent that can be used to stain cells and nucleic acidsPI binds to DNA by intercalating between the bases with little or no sequence preference. Excitation filter Dichroic mirror Emission filter Remarks. Thus with an appropriate mixture of the SYTO 9 and propidium iodide stains bacteria with intact cell membranes ie live stain fluorescent green whereas bacteria with damaged membranes ie dead stain fluorescent red. Excitation and Emission Peaks Dye Excitation Max nm Emission Max nm 6-Carboxyrhodamine 6G 525 555 7-Aminoactinomycin D 7-AAD 546 647 7-Hydroxy-4-methylcoumarin 360 455 Acid Fuchsin 540 630 Acridine orange DNA 500 526 Acridine orange RNA 460 650 Acridine Red 455-600 560-680 Acridine Yellow 470 550.
The SYTO green-fluorescent nucleic acid stains have prov en valuable in a broad range of research applications.
Propidium IodideSyto 9 FLUOROPHORES GUIDE L I V E D E A D B A C L I G H T B A C T E R I A L V I A B I L I T Y K I T S Syto 9 Excitation Syto 9 Emission Optical Kit Excitation Optical Kit Emission 400 500 600 700 Propidium Iodide Excitation Propidium Iodide Emission Optical Kit Excitation Optical Kit Emission 400 500 600. SYTO9 The Mix contains fluorescent DNA dye SYTO9 which after binding to doublestranded dsDNA becomes strongly fluorescent with maximal excitation at 485 nm and emission at 498 nm green light. OsII complexes the wavelength of excitation was 475 nm and the emission was measured at 745 nm. 47040 450490 495 LP 52550 500550 Wide Excitation band Bandpass Emission. Equipment 2 Fluorescence Spectroscopy. The manufacturer rec-ommends that PI should be used at a concentration six times that of SYTO9 in initial trials with the kit.