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Aug
Two Photon Action Cross Section. Values for the two-photon absorption cross section are derived by comparing the fluorescence signals excited by wavelengths of 800 and 400 nm using a second-harmonic generator. I have large two-photon action cross section TPACS which is the product of two-photon absorption cross section σ 2PA and fluorescence quantum yield Φ exceeding 50 GM. We report on a novel method designed for measuring two-photon action cross sections spectra in a single shot without tuning the excitation wavelength. 3-photon excitation of GCaMP6.
Excitons in semiconductor quantum dots QDs feature high values of the two-photon absorption cross-sections TPACSs enabling applications of two-photon-excited photoluminescence TPE PL of QDs in biosensing and nonlinear optoelectronics. We report on a novel method designed for measuring two-photon action cross sections spectra in a single shot without tuning the excitation wavelength. Moreover as the two-photon excited fluorescence applied in bioimaging typically. Photosensitivity is quanti-fied as the uncaging action cross section du which is the product of the two-photon absorbance cross section da and the uncaging. I have large two-photon action cross section TPACS which is the product of two-photon absorption cross section σ 2PA and fluorescence quantum yield Φ exceeding 50 GM. Values for the two-photon absorption cross section are derived by comparing the fluorescence signals excited by wavelengths of 800 and 400 nm using a second-harmonic generator.
Measurements were performed by use of ultrashort 100-fs Tisapphire pulsed excitation over the range 690960 nm.
For that purposes two-photon probes should fulfill several requirements. TPE spectra were obtained by comparison with a fluorescein calibration standard. Measurements were performed by use of ultrashort 100-fs Tisapphire pulsed excitation over the range 690960 nm. From the two-photon fluorescence signal a TPE ac-tion cross section s TPE can be determined. 3-photon excitation of GCaMP6. For determining two-photon cross sections is to mea-sure the fluorescence signal generated by TPA.